Micropropagation of Hemp (Cannabis sativa L.)

Hemp ( Cannabis sativa L.) is commonly grown for the medicinal secondary metabolites produced by pistillate inflorescences. Micropropagation a valuable method of propagating hemp plants because aseptic process and production true-to-type propagules. The cultivar TJ’s CBD was used series experiments to compare media inputs practices clonal micropropagation hemp. For stage I, shoot tips harvested from stock that were in growth chamber less endogenous contamination newly established cultures than greenhouse. In addition, I disinfection treatments with 20%, 40%, 60% bleach (7.5% sodium hypochlorite) 10 minutes had no differences surface rates. All concentrations able clean explants equally, damage observed. II, there multiplication rate between tip or nodal explants. observed gelling agent’s agar, agargellan, gellan gum at standard When basal nutrient formulations compared rates their respective vitamins, Murashige Skoog, Linsmaier & Driver Kuniyuki Walnut found be superior Lloyd McCown Woody Plant Medium. Media pH levels 4.0, 5.0, 5.8, 6.0, 7.0 compared, final fresh weights, lengths, quality ratings. generated greater number lateral nodes. Sucrose 0%, 1.5%, 3.0%, 4.5%, 6.0% (wt/vol) also 1.5% 3.0% showing Growth room temperatures 22, 24, 26, 28 °C 26 generating numbers nodes, ratings cooler temperatures. cytokinins 6-enzylaminopurine (BA), 6-(γ,γ-dimethylallylamino) purine (2iP), thidiazuron (TDZ) 1.0, 10.0 μM concentrations. 5.0-μM TDZ treatment weights nodes; however, it shortest lengths lowest 2iP 1.0 5.0 μM, BA greatest level considered best II based on high ratings, addition nodes produced. III experiments, auxins indole 3-butyric acid (IBA) 1-naphthylacetic (NAA) 0.25, 0.5, 2.5 μM. Auxin 0.25 NAA, 0.5 IBA root However, 2.5-μM resulted higher overall rating. IV, ex vitro rooting acclimation trials dome an intermittent mist system, as well treated unrooted cuttings externally applied auxin. Acclimating heights, system. auxin mildly increased weight, but not important success domed environment. It has been concluded system uses lower sucrose, growing temperatures, are optimal when acclimating tissue culture, can bypassed rooted during IV without additional treatments.